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| The Correlations of Parameters Using Contrast Enhanced
Ultrasonography in the Evaluation of Prostate Cancer
Angiogenesis |
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| Sung Il Hwang, MD1 Hak Jong Lee, MD1-3, Kil Joong Kim, PhD1,
Jin-haeng Chung, MD4, Hyun Sook Jung, PhD4, Jong June Jeon, PhD5 |
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| 1Department of Radiology, Seoul National University Bundang Hospital
2Program in Nano Science and Technology, Department of Transdisciplinary Studies, Seoul
National University Graduate School of Convergence Science and Technology
3Nano-imaging and Therapy Research Center, Advanced Institutes of Convergence
Technology, Seoul National University
4Department of Pathology, Seoul National University Bundang Hospital
5Department of Statistics, Seoul National University |
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| Purpose: The purpose of this study is to investigate the correlations of various
kinetic parameters derived from the time intensity curve in a xenograft mouse model
injected with a prostate cancer model (PC-3 and LNCaP) using an ultrasound contrast
agent with histopathologic parameters.
Materials and Methods: Twenty nude mice were injected with human prostate
cancer cells (15 PC-3 and five LNCaP) on their hind limbs. A bolus of 500 μL (1 × 108
microbubbles) of second-generation US contrast agent (SonoVue) was injected into
the retroorbital vein. The region of interest was drawn over the entire tumor. The time
intensity curve was acquired and then fitted to a gamma variate function. The maximal
intensity (A), time to peak (Tp), maximal wash-in rate (washin), washout rate
(washout), area under the curve up to 50 sec (AUC50), area under the ascending
slope (AUCin), and area under the descending slope (AUCout) were derived from the
parameters of the gamma variate fit. Immunohistochemical staining for VEGF and
CD31 was performed. Tumor volume, the area percentage of VEGF stained in a field,
and the count of CD31 (microvessel density, MVD) positive vessels showed correlation
with the parameters from the time intensity curve.
Results: No significant differences were observed between the kinetic and
histopathological parameters from each group. MVD showed positive correlation with
A (r=0.625, p=0.003), washin (r=0.462, p=0.040), AUC50 (r=0.604, p=0.005), and
AUCout (r=0.587, p=0.007). Positive correlations were also observed between tumor
volume and AUC50 (r=0.481, p=0.032), washin (r=0.662, p=0.001), and AUCout
(r=0.547, p=0.012). Washout showed negative correlations with MVD (r=
-0.454, p=0.044) and tumor volume (r=-0.464, p=0.039). The area percentage of
VEGF did not show any correlation with calculated data from the curve.
Conclusion: MVD showed correlations with several of the kinetic parameters. CEUS
has the potential for prediction of tumor vascularity in a prostate cancer animal
model. |
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| Keyword : Ultrasonography; Contrast agent; Prostate cancer; Parametric imaging; |
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pdf파일 : 132-142황성일.pdf 
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> Journal > Journal Search 
Journal of Korean Society of Ultrasound in Medicine 2013 ; 32 (2) : p.132-142
